Transcriptome is the complete set of transcripts from genome transcription in a specific condition. Thus, RNA-seq is the accurate and highly sensitive method of choice to measure gene expression level and to compare it between different samples and conditions. With this tool, it is also possible to analyze novel transcripts, isoforms, rare transcripts, SNVs variants, gene splicing and gene fusion.
What BIOfidal can do for you?
Depending on your organism of interest, BIOfidal can sequence your RNA-seq project on MiSeq system with flexibility based on multiple approaches: rRNA depletion, mRNA selection, strand-specific sequencing or not, single read or paired-end run sequencing, according to your need.
What about the bioinformatics?
A highly accurate analysis pipeline is available for the quantitation of expression levels of annotated transcripts by mapping the reads on the reference and calculating the RPKM values of each sample. The expression level comparison between different groups can lead to a statistical differential analysis.
What you need to provide us?
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Favre-Bonté S et al. Analyse transcriptomique par RNA-seq de la réponse de Stenotrophomonas maltophilia à l’exposition à des stress abiotiques et chimiques. Poster session presented at: Journée d’échanges de la FR BioEnviS, Villeubanne. 2016 Oct 27