DNA sequencing provider Lyon : Post PCR analysis reagents for electrophoresis

Post PCR analysis reagents for electrophoresis

 

 

 

GenAgarose

 

GenAgarose is suitable for all analytical and preparative electrophoresis of nucleic acids in routine gel electrophoresis. Depending on the concentration of GenAgarose used, the size range of nucleic acid separation will vary between 0.2 and 15 kb. The low EEO makes this useful for a broad range of applications: PCR product analysis, restriction enzyme digest analysis, separation of RNA before blotting, etc.

 

Products Cat. No. Pack Size List price €HT
GenAgarose GX04002 100 g 56.00
GX04003 250 g 126.50
GX04004 500 g 195.00

 

 

 


 

 

 

Electrophoresis buffers

All our buffers are Molecular Biology Grade

 

 

50× and 10× TAE

10× and 50× TAE buffer are sterile filtered solution of Tris-Acetate and EDTA. TAE buffer is the most commonly used buffer for agarose DNA electrophoresis. A 1× TAE buffer solution contains 40 mM Tris-Acetate and 1 mM EDTA at pH 8.3.

Products Cat. No. Pack Size List price €HT

TAE buffer (10×)

Ready-to-use solution

GX12760 1 L 13.00
GX12761 5 L 39.00

TAE buffer (10×)

Dry Powder Pack, for 1 L of 10×

GX12762 2 packs 45.00

TAE buffer (50×)

Ready-to-use solution

GX12763 500 ml 14.00
GX12764 1 L 29.00

 

 

Tris-Borate-EDTA (TBE) Buffer

 

TBE is optimized for DNA purification and DNA sequencing techniques using both acrylamide and agarose. BIOfidal offers TBE in a variety of concentrations and packaging options for maximum flexibility and convenience. Packaging options includes liquid concentrate, and Ready-Pack™ (a foil pouch containing sufficient material to prepare 1L of 10× concentrate).

 

Products Cat. No. Pack Size List price €HT

TBE buffer (10×)

Ready-to-use solution

GX12772 1 L 13.00
GX12773 5 L 45.00

TBE buffer (10×)

Dry Powder Pack, for 1 L of 10×

GX12774 2 packs 45.00

 

 

 


 

 

 

DNA ladders

 

 

GenLadder 100 bp Ready-to-load

GenLadder 100 bp DNA Ladder is a ready-to-load molecular weight marker suitable for DNA fragment size determination on gel electrophoresis. The 100 bp DNA Ladder is formulated to run accurately and to provide crisp band patterns. It contains bromophenol blue dye which serves as visual aid to monitor the progress of migration during agarose gel electrophoresis. The 100 bp DNA Ladder contains 12 discrete DNA fragments ranging from 100 bp to 3,000 bp.

To view its profil.

 

Products Cat. No. Pack Size List price €HT

GenLadder 100 bp

Ready-to-load

GX04041 50 µg 35.00
GX04042 5 × 50 µg 149.00

 

 

GenLadder 1 kb Read-to-load

 

GenLadder 1 kb DNA Ladder is formulated to run accurately and to provide crisp band patterns. It contains two dyes: bromophenol blue and xylene cyanole which serve as visual aid to monitor the progress of migration during agarose gel electrophoresis. The 1 kb DNA Ladder contains 13 discrete DNA fragments ranging from 300 bp to 10,000 bp.

To view its profil.

 

Products Cat. No. Pack Size List price €HT

GenLadder 1 kp

Ready-to-load

GX04045 50 µg 32.00
GX04046 5 × 50 µg 139.00

 

 

 


 

 

 

Loadings Dyes and Stains

 

 

Ethidium Bromide 1%

 

Ethidium Bromide (EtBr) is the most commonly used fluorescent dye for labelling of dsDNA and RNA in agarose gel electrophoresis.

 

Products Cat. No. Pack Size List price €HT

Ethidium Bromide 1%

Aqueous solution (w/v)

GX12797 10 ml 18.00

 

 

DNA loading dyes

 

6× DNA Loading Dye Buffers can be used for loading DNA samples (PCR products, restriction fragments) on agarose or polyacrylamide gel.

 

Products Cat. No. Pack Size List price €HT

6× DNA Loading Dye Buffer Blue

Bromophenol Blue

GX02042 5 × 1 ml 33.00

6× DNA Loading Dye Buffer Double Blue

Xylene Cyanole FF, Bromophenol Blue

GX02046 5 × 1 ml 33.00

6× DNA Loading Dye Buffer Orange

Xylene Cyanole FF, Orange G

GX02048 5 × 1 ml 33.00