DNA sequencing provider Lyon : HOT BIOAmp® Evagreen qPCR mix

HOT BIOAmp® Evagreen qPCR mix

HOT BIOAmp® EvaGreenqPCR Mix is an optimized ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR: HOT BIOAmp® DNA Polymerase, ultrapure dNTPs, MgCl2 and EvaGreen® dye. The user simply needs to add water, template and primers. HOT BIOAmp® EvaGreen qPCR mix exists with no ROX and high ROX.

HOT BIOAmp® DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

 

Applications:

-  Detection and qualification of DNA and cDNA targets

-  Profiling gene expression

-  Microbial detection

-  Viral load determination

-  High Resolution Melt (HRM) Analysis.

 

 

HOT BIOAmp® Evagreen qPCR mix is declined in a version HOT BIOAmp® EvaGreen® qPCR Supermix, wich have many benefits :

- Highly specific and reproducible real time PCR

- Excellent efficiency in case of low copy number targets

- UNG treatment capability due to dNTP blend of dUTP/dTTP

- Superior performance with long (up to 500 bp) and GC-rich templates

- Blue visualisation dye for easy pipetting.

 

 

Products Cat. No. Pack Size List price €HT
5× HOT BIOAmp® Evagreen qPCR Mix (high ROX) GX02050 1 ml 65.00
GX02051 8 ml 450.00
5× HOT BIOAmp® Evagreen qPCR Mix (no ROX) GX02052 1 ml 65.00
GX02053 8 ml 450.00
5× HOT BIOAmp® EvaGreen® qPCR Supermix GX02054 1 ml 85.00
GX02055 8 ml 570.00

 

qPCR platforms compatibility

 

 

 

 

Your publications

Hammed A et al. J Biol Chem. 2013 Oct 4; 288 (40): 28733 - 42

Lefebvre S et al. Toxicology. 2016 Feb 17; 344 - 346: 34 - 41

Boitet M et at. Pest Manag Sci. 2017 Nov 20

Espana B et al. Biopharm. Drug Dispos. 2017; 38: 20–32